Automated DNA/RNA purification (96 well)
The adaptation of well established STRATEC Molecular HTS kits to common robotic workstations allows for a simple and convenient automated isolation of DNA and RNA from different starting materials. Procedures previously carried out manually can now be easily and quickly performed.
Advantages:
- automated nucleic acid isolation – up to 96 samples per run
- cross contamination free procedures
- purification of high-quality, ready-to-use nucleic acids
- ready-made and customized purification protocols
- high binding capacity and recovery values of membranes
- protocols adapted to different robotic platforms
Kit Overview
| Sample Source | Recommended Kit | Sample Volume / well | Yield / well |
| Plasmid DNA Purification | |||
| Bacterial suspension, bacteria pellets | Invisorb Plasmid HTS 96 Kit/ R | 0.5 - 1.0 ml | 10 μg |
| Genomic DNA Purification | |||
| Whole human or animal blood, buffy coat | Invisorb Blood Mini HTS 96 Kit/ X | up to 200 µl | 2 - 10 µg |
| Human or animal tissue, rodent tails, paraffin embedded tissue, mammalian cells | Invisorb DNA Tissue HTS 96 Kit/ Tec | up to 20 mg tissue, 0.5 - 0.8 cm rodent tails up to 106 cells | 10 - 25 μg |
| Nucleic Acid Purification from Pathogens | |||
| Bacterial DNA | |||
| Serum, plasma, whole blood, cell-free body fluids, swabs, bacterial cultures, tissue material | Invisorb Universal Bacteria DNA HTS 96 Kit/ R | up to 100 µl 5 - 10 mg | depending on starting material |
| Viral DNA | |||
| Plasma, serum, whole blood,cell-free body fluids, rinse liquid from swabs | Invisorb Virus DNA HTS 96 Kit/ R | up to 200 µl | depending on starting material |
| Viral RNA | |||
| Plasma, serum, cell-free body fluids, rinse liquid from swabs, stool samples | Invisorb Virus RNA HTS 96 Kit/ R | up to 200 µl | depending on starting material |
Automation resources - implementation on robotic workstations
STRATEC Molecular offers a variety of kits for full automation based on different technologies (membrane, magnetic beads). Long years of experience in kit development and optimization in combination with a thorough understanding of requirements, chances and limitations in automation have resulted in highly optimized solutions for automated needs. A team of experienced application specialists will perform the integration of the HTS 96 kit on the customer’s robotic workstation, either.
- in collaboration with the customers themselves or
- with specialists from the producer of the laboratory workstations.