PCR Product & DNA Fragment Purification and Concentration

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PCR Product & DNA Fragment Purification and Concentration

Advantages

convenient and fast sample processing
the most efficient removal of contaminants
high recovery rate of PCR products or DNA fragments
broad range of fragment sizes 80 bp - 30 kb can be purified

Kit Overview

Sample Source	Recommended Kit	Sample Volume	Yield
PCR products, ligation reaction mixture, enzyme digestion mixture, cDNA synthesis mixture, cycle sequencing reaction mixture	MSB Spin PCRapace Invisorb Fragment CleanUp	up to 100 μl	80 - 95 % *
Highly contaminated DNA, DNA fragments and PCR products	Invisorb DNA CleanUp	up to 200 μl	60 - 85 % *
Agarose gels (TAE, TBE)	Invisorb Spin DNA Extraction Invisorb Fragment CleanUp	up to 400 mg gel slices	70 - 90 % **

* dependent on fragment length

** dependent on fragment length and kind of agarose gel

The purification procedure has been optimized to remove salts, enzymes, nucleotides, mineral oil, agarose, ethidium bromide and other impurities from DNA samples. Specialized binding buffers promote selective adsorption of DNA fragments and PCR products. The pure DNA is eluted in a small volume of buffer or water, ready to use for any subsequent application.

^{The MSB and Invisorb technology for purification of PCR products and DNA fragments is covered by patents and patent applications: DE10253 351.2 ,EP0765335B1, DE4422044A1, US6,037,465, EP1135479B1, DE19856064C2, DE19856064A1, EP01964906.0, DE10041825 A1, US5,141,719, DE10233790A1.}

DNA Fragment Purification