Genomic DNA Isolation
The genomic DNA binds under non-chaotropic conditions to the surface of the different supports. All impurities are very efficiently removed and the ultra-pure DNA is eluted in a low-salt buffer. The kits provide reproducible yields of highly purified genomic DNA and allow for the use of the purified nucleic acid in downstream application without any risk.
Advantages:
- high-quality genomic DNA without toxic chaotropic reagents in minutes
- avoids interference by contaminating salts
- easy procedure
- requires no handling of potentially hazardous buffers
- ready to use DNA for any downstream application and long term storage
- high yields even from difficult and small amounts of starting material
Kit Overview
| Sample Source | Recommended Kit | Sample Volume | Yield |
| Whole blood and buffy coat | Invisorb Spin Micro DNA Kit Invisorb Spin Blood Mini Kit Invisorb Spin Blood Midi Kit Invisorb Spin Blood Maxi Kit Invisorb Blood Universal Kit | 1 - 50 μl 1 - 200 µl 200 μl - 2 ml 1 - 10 ml 1 ml - 10 ml | 1 - 2 μg up to 10 µg up to 60 μg up to 300 μg up to 400 μg |
| Body fluids, bone marrow | Invisorb Spin Micro DNA Kit Invisorb Spin Blood Mini Kit Invisorb Genomic Kit III | 1 - 50 μl 1 - 200 µl 1 - 200 µl | depending on type and amount of starting material |
| Human and animal tissue, paraffin embedded tissue | Invisorb Spin Micro DNA Kit Invisorb Spin Tissue Mini Kit Invisorb Spin FFPE Tissue Kit Invisorb Genomic Kit II | 0.5 - 5 mg 0.5 - 40 mg 2 - 3 paraffin sections 0.5 - 40 mg | 1 - 2 μg up to 50 μg up to 50 μg up to 50 μg |
| Human and animal cells | Invisorb Spin Micro DNA Kit Invisorb Spin Tissue Mini Kit Invisorb Genomic Kit III Invisorb Spin Cell Mini Kit | 100 -105cells 10 –107cells 1 x 105 cells 2 x 106 cells | depending on type and amount of starting material |
| Yeast cells | Invisorb Spin Yeast DNA Mini Kit Invisorb Genomic Kit III | 107 cells, swab 3 - 10 ml bacterial or yeast suspension | depending on type of starting material |
| Swab material | Invisorb Spin Swab Kit Invisorb Genomic Kit III Invisorb Spin Cell Mini Kit | depending on starting material | depending on starting material |
| Forensic material | Invisorb Spin Forensic Kit Invisorb Forensic Kit I | depending on type and amount of starting material | depending on type and amount of starting material |
| Plant material | Invisorb Spin Plant Mini Kit | up to 100 mg | 5 - 35 μg |
| Food from animal / plant origin | Invisorb Spin Food Kit II (plant) | up to 100 mg | up to 50 μg |
Products which are CE-marked according to the IVD-Directive (Directive 98/79/EC on in vitro diagnostic medical devices) can be used for diagnostic applications in countries where this directive is recognized.
The Invisorb technology for the isolation of genomic DNA is covered by patents or patent applications: EP1135479 B1, DE19856064C2, DE19856064A1, EP0765335B1, DE4422044A1,US 6,037,465, EP01964906.0, DE1004825A1, US5,141,719, DE10233790 A1