Invisorb® Spin Virus RNA Mini Kit

Use the Invisorb Spin Virus RNA Mini Kit for reliable isolation of high-quality RNA from viruses found in a diverse range of starting materials. The kit simplifies viral RNA isolation by combining efficient lysis of the starting material and the inactivation of exogenous and endogenous RNases to prevent degradation

Starting Material

cell-free body fluids of human origin, whole blood, swab material, rinse liquid from swabs, cell culture supernatants, mammalian cells, tissue samples, stool samples

Product Characteristics

Advantages

• RT-PCR templates in only 20 minutes with high sensitivity at low virus titers
• for various sample types
• complete removal of PCR inhibitors
• safe handling of infectious samples by using capped spin columns
• CE-marked in compliance with the Directive 98/79/EC on in vitro diagnostic medical devices (IVD-Directive)*

Overview

The sample is lysed in an optimized lysis buffer and proteins are degraded during the lyses with Proteinase K. To guarantee RNA integrity, samples are lysed under highly denaturing conditions to inactivate RNases. The addition of Carrier RNA is necessary for the enhancement of DNA recovery in samples with low virus titer. Carrier RNA also stabilizes nucleic acids in samples with very small nucleic acid concentrations. The liberated RNA is bound onto the membrane of the SafeClick RTA Spin Filter. Contaminants are removed by repeated washing steps and the purified viral RNA can be eluted in a small volume of RNase-free water. Due to the high purity, the isolated viral RNA is ready to use for a broad panel of downstream applications like:

• RT-PCR
• TaqMan analysis and array technologies
• RNA dot blots
• cDNA transcription
• in vitro translation

*) Products which are CE-marked according to the IVD-Directive can be used for diagnostic applications in countries where this directive is recognized.

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