RTP® Mycobacteria Kit

The RTP Mycobacteria Kit allows for the rapid and efficient isolation of high quality DNA from Mycobacteria species from sputum, bronchoalveolar lavage samples and small tissue samples.

Starting Material

sputum, bronchial secretions (BALB), small tissue biopsy sample (like lymph nodes)

Product Characteristics

Advantages

• highly efficient recovery of high-quality bacterial DNA, allowing for very sensitive pathogen detection
• using pre-filled Extraction Tubes containing lyophilized lysis components (lysis buffer, lysozyme, Proteinase K) and Carrier RNA
• special buffer for reduction of the sample viscosity is provided with the kit
• CE-marked in compliance with the Directive 98/79/EC on in vitro diagnostic medical devices (IVD-Directive)*

Overview

The specially designed kit includes NAC Buffer for the standard pretreatment of 200 μl – 5 ml sputum samples before lysis of the material. The resulting bacterial cell pellet is dissolved in a special Resuspension Buffer and completely transferred to the Extraction Tube. Optimized buffer and enzymes lyse the samples under a special temperature profile, stabilize nucleic acids and selectively enhance DNA adsorption to the membrane. The membranes are efficiently washed before the nucleic acids are eluted in order to remove all PCR inhibitors and proteins. Based on patented technology, the kit uses buffers without any hazardous chaotropic components. The purified high quality DNA is ready to use for subsequent downstream applications or can be stored at –20 °C for subsequent use:

• PCR
• real-time PCR (quantitative PCR, like TaqMan and LightCycler technologies)
• restriction digestion
• RFLP analysis

*) Products which are CE-marked according to the IVD-Directive can be used for diagnostic applications in countries where this directive is recognized.

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