RTP® Pathogen Kit

The RTP Pathogen Kit provides a fast and efficient way for reliable simultaneous isolation of high quality bacterial DNA, viral DNA and RNA from a diverse range of starting materials.

Starting Material

serum, plasma, other cell-free body fluids, cell culture supernatants, rinse liquid from swabs, tissue biopsies, bacterial cells

Product Characteristics

ProductStarting MaterialAverage YieldPreparation Time
RTP® Pathogen Kitup to 200 μl serum, plasma, cell-free body fluids, cell culture supernatant
up to 10 mg tissue samples
up to 400µl rinse liquid from swabs
109 bacteria cells
depending on starting material and viral titer20 min

Advantages

  • highly efficient recovery of high-quality bacterial DNA, viral DNA and RNA from different sources, allowing for very sensitive bacteria and virus detection
  • remarkable reduction of handling steps and processing time
  • pre-filled Extraction Tube contains lyophilized lysis components (lysis buffer, lysozyme, Proteinase K) and Carrier RNA
  • CE-marked in compliance with the Directive 98/79/EC on in vitro diagnostic medical devices (IVD-Directive)*

Overview

Using the RTP Pathogen Kit all types of samples are transferred to the Extraction Tube together with a specially designed Resuspension Buffer. The pre-filled buffer and enzymes lyse the samples, stabilize the nucleic acids, inactivate RNases and DNases and enhance the viral and bacterial DNA and/ or RNA adsorption to the membrane of the SafeClick Spin Filter. Contaminants are removed by repeated wash steps and the purified nucleic acids can be eluted in a small volume of Elution Buffer.
By using the pre-filled Extraction Tubes, the preparation of carrier nucleic acid solution or enzyme solution, as well as the pipetting of different components to the sample during the lysis, are not longer necessary. This reduces handling steps with infectious samples, processing time and contamination risk to a minimum.

*) Products which are CE-marked according to the IVD-Directive can be used for diagnostic applications in countries where this directive is recognized.

Ordering Information

Product Package Size Catalogue No
RTP Pathogen Kit50 purifications1040500200
RTP Pathogen Kit250 purifications1040500300

Process Overview

Applications

Manuals

Manual

MSDS download

MSDS
MSDS
MSDS
MSDS
MSDS
MSDS