Total RNA Isolation
A unique feature of the STRATEC Molecular RNA Kits is the selective removal of genomic DNA from the lysate before RNA purification This step eliminates the hassle and cost of DNase digestion, which is generally required with kits from other suppliers to avoid interference of DNA in RT-PCR reactions.
Advantages:
- high-quality total RNA from different starting materials
- DNA removal without expensive DNase digestion
- time saving and convenient handling
- high, reproducible yields
- accurate, reliable gene expression data
- versatile use - parallel RNA and protein isolation, RNA and DNA isolation, RNA CleanUp from Trizol phases
Kit Overview
| Sample Source | Recommended Kit | Sample Volume | Yield |
| Human and animal cells | InviTrap Spin Cell RNA Mini Kit InviTrap Spin Universal RNA Mini Kit | up to 107cells | up to 150 µg |
| Human and animal tissue | InviTrap Spin Tissue RNA Mini Kit InviTrap Spin Universal RNA Mini Kit | up to 20 mg up to 20 mg | up to 100 µg |
| Bacteria and yeast | InviTrap Spin Cell RNA Mini Kit | up to 109 bacterial cells, up to 5 x 107 yeast cells | depending on type and amount of starting material |
| Whole blood | InviTrap Spin Blood RNA Mini Kit InviTrap Spin Universal RNA Mini Kit | up to 1.5 ml | up to 1 - 5 µg total RNA / ml of whole blood |
| Serum and plasma | Invisorb Spin Virus RNA Mini Kit | up to 200 µl | depending on viral titer |
| Plant cells and tissue, filamentous fungi | InviTrap Spin Plant RNA Mini Kit | 10 – 100 mg fresh plant tissue, up to 107 plant or fungal cells | up to 100 µg |
Workflow
The InviTrap RNA kits combine an efficient, stringent lysis and an inactivation of endogenous and exogenous RNases with the selective removal of genomic DNA by binding at the surface of the mineral carrier particles provided within the provided Lysis Solutions and simultaneously to the lysis of the starting material. Selective binding of the DNA is assured by optimized buffer conditions and the mineral carrier particles loaded with the genomic DNA are removed by spin down or by centrifugation on the spin filter surface. The RNA remains in the supernatant in the filtrate and is - after the adjustment of special binding conditions- bound onto the membrane of the RNA-RTA Spin Filter. Contaminants are removed by repeated washing steps and the purified total RNA which is almost free of DNA contaminations can be eluted in a small volume of RNase free water.
Products which are CE-marked according to the IVD-Directive (Directive 98/79/EC on in vitro diagnostic medical devices) can be used for diagnostic applications in countries where this directive is recognized.
The InviTrap technology for isolation of total RNA is covered by following patents and patent applications: EP 0 880 535 B1, DE 195 06 887 C 2, US 6,110,363, DE 44 47 015 C2, DE 44 47 015 A1)